Book chapter on how to smFISH in zebrafish embryos
Interested in detecting single transcripts from zebrafish embryos? or applying smFISH to your research? Carine’s chapter in Methods in Molecular Biology is out!
The chapter provides a detailed protocol: from setting up the embryos to cryosection, to smFISH, to image analysis. You’ll also find a description of the pipeline for membrane segmentation and transcription detection. Visit Book Chapter at Methods in Molecular Biology